Journal: bioRxiv

Article Title: Ex-vivo mouse precision cut tumour slices for modelling hepatocellular carcinoma; A 3Rs solution for at-scale drug screening

doi: 10.1101/2024.09.16.613213

Figure Lengend Snippet: (A) The key details the panel of 26 therapeutic molecules used in PCTS drug screen and the concentrations that they were applied at. (B) Schematic shows the timeline of 3mm PCTS generation and subsequent 8-day culture period in static 96-well culture plates, with 26 drugs from the screening panel applied from day 1 onwards. (C-D) Graphs show day 8 (C) metabolic activity and (D) ATP production from PCTS cultured with 26 therapies from the screening panel applied at two doses, measured via resazurin assay and CellTiter-Glo assay respectively. Data are mean ± s.e.m. for N=6 PCTS from N=5 tumours. (E) Graph shows correlation between ATP production and metabolic activity, measured by CellTiter-Glo assay and resazurin assay respectively, from PCTS cultured with 26 therapies from the screening panel applied at two doses, with emphasis on rottlerin, salinomycin and sorafenib. (F-J) Graphs show luciferase assay data from PCTS cultured with vehicle control and two doses of (F) sorafenib, (G) lenvatinib, (H) anti-PD1, (I) rottlerin and (J) salinomycin. Data are mean ± s.e.m. from N=6 PCTS from N=5 tumours. (K) Schematic shows the timeline of intrahepatic transplant of Hep-53.4 cells to generate orthotopic tumours, followed by therapeutic intervention at day 14 with salinomycin (4 mg/kg) or vehicle control via daily intraperitoneal injection, before tumours were harvested at day 28. (L) Graph shows tumour burden of Hep-53.4 tumours treated with vehicle control or salinomycin (4 mg/kg). Data are mean ± s.e.m. from up to N=7 mice per treatment group.

Article Snippet: Therapeutic intervention with 4 mg/kg salinomycin (MedChemExpress) or a corn oil/DMSO control was started 14 days post implantation was administered via daily intraperitoneal injection until mice were harvested at day 28.

Techniques: Activity Assay, Cell Culture, Resazurin Assay, Glo Assay, Luciferase, Control, Injection

Journal: bioRxiv

Article Title: Ex-vivo mouse precision cut tumour slices for modelling hepatocellular carcinoma; A 3Rs solution for at-scale drug screening

doi: 10.1101/2024.09.16.613213

Figure Lengend Snippet: (A) Graph shows day 8 secreted luciferase levels from PCTS cultured with 26 therapies from the screening panel applied at two doses, measured via Nano-Glo luciferase assay. Data are mean ± s.e.m. for N=6 PCTS from N=5 tumours. (B) Schematic shows the timeline of PCLS and PCTS generation and subsequent culture period of 4 and 8 days respectively. (C-D) Graphs show metabolic activity of (C) PCTS and (D) PCLS cultured for 8 and 4 days respectively with vehicle control, sorafenib (20 µM) or salinomycin (5 µM – 10 µM), measured via resazurin assay. Data are mean ± s.e.m. from up to N=4 PCTS/PCLS per treatment group and time point. (E) Graph shows liver to body weight ratio of mice with Hep-53.4 orthotopic tumours following treatment with vehicle control or salinomycin (4 mg/kg). Data are mean ± s.e.m. from up to N=8 mice per treatment group.

Article Snippet: Therapeutic intervention with 4 mg/kg salinomycin (MedChemExpress) or a corn oil/DMSO control was started 14 days post implantation was administered via daily intraperitoneal injection until mice were harvested at day 28.

Techniques: Luciferase, Cell Culture, Activity Assay, Control, Resazurin Assay

Journal: PLoS ONE

Article Title: Determination of regulatory ionophore coccidiostat residues in feedstuffs at carry-over levels by liquid chromatography-mass spectrometry

doi: 10.1371/journal.pone.0182831

Figure Lengend Snippet: Chromatogram of representative blank feed spiked at the following concentrations: 1.25 mg kg -1 for nigericin, 0.125 mg kg -1 for semduramicin sodium, 0.625 mg kg -1 for lasalocid sodium, 0.350 mg kg -1 for salinomycin sodium, 0.625 mg kg -1 for monensin sodium, 0.350 mg kg -1 for narasin, 0.025 mg kg -1 for maduramicin ammonium.

Article Snippet: Salinomycin sodium (SAL), monensin sodium (MON), and only lasalocid sodium (LAS) as solution at 100 μg ml -1 in acetonitrile, were purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany).

Techniques:

Journal: Translational Animal Science

Article Title: Effects of amino acid biomass or feed-grade amino acids on growth performance of growing swine and poultry 1 2

doi: 10.1093/tas/txz163

Figure Lengend Snippet: Male broiler starter and grower diet composition, experiment 2 (as-fed basis) 1

Article Snippet: 6 Active drug ingredient salinomycin sodium, 132 g/kg (66 mg/kg inclusion; Huvepharma, Peachtree City, GA).

Techniques:

Journal: Translational Animal Science

Article Title: Effects of amino acid biomass or feed-grade amino acids on growth performance of growing swine and poultry 1 2

doi: 10.1093/tas/txz163

Figure Lengend Snippet: Male broiler starter and grower diet composition, experiment 3 (as-fed basis) 1

Article Snippet: 6 Active drug ingredient salinomycin sodium, 132 g/kg (66 mg/kg inclusion; Huvepharma, Peachtree City, GA).

Techniques: